Methods and Solutions for Tissue Preservation

Tech Id: kam000030

 

Introduction

The supply of high quality organs has always been one of the most important steps in organ transplantation. In 2015, there were over 2,500 heart transplants performed in the US and nearly 4,200 people on the waiting list. A significant obstacle to successful heart transplantation is that the tolerated ischemia time of a heart is limited to about 6 hours with currently available preservation methods. This time frame also restricts the geographic region from which a donor organ can be transported before the viability is compromised. There is a need for an improved cardiac transplantation solution that enables the prolonged organ storage. Prolonged tolerable ischemia time could improve organ transplantation quality and increase the number of potential donors.

 

Technology Description

Dr. Kamlot and colleagues at Cedars-Sinai Medical Center have invented a new composition for an aqueous tissue preservation solution which has application is preserving heart, liver, pancreas, kidney or lung for transplantation. The inventive composition is hyperoxygenated and is formulated at a low pH of 6.5-7 rather than the physiological pH of 7.2-7.4 which is common in currently available preservation solutions. The significant quantity of dissolved oxygen allows for decreased ischemia and longer storage time. The preservation solution also includes rapidly available energy sources, energy transport shuttles, and free radical scavengers. Together with the low pH, these minimize the damage done by free radicals and protect against the loss of cell viability and membrane degradation. The inventors performed a comparative study of donor organ function in transplantation preservation solution where the use of inventive composition demonstrated significant (i.e. about 70% to 130%) improvement over UW solution in preserving hearts of rats.

 

Advantages/Novelty

• Very inexpensive to produce since only small amounts of basic components are used.

• Hyperoxygenated solution allows for decreased ischemia and longer storage time.

• Energy source in low pH protects against the loss of cell viability and membrane degradation.

• Radical scavengers protect cellular structures against oxidative damage from radicals.

 

Applications 

This preservation solution can improve heart tissue preservation during cardiac transplantation. It might also be a valuable solution for other organ transplants or for organ transport. 

 

Intellectual Property

 

US 8,900,804 B2

Patent Information:
Title App Type Country Serial No. Patent No. File Date Issued Date Expire Date
Methods and Solutions for Tissue Preservation Provisional USA 60/630,118 11/22/2004   11/22/2005
Cardiac Transplantation Preservation Solution Patent Cooperation Treaty WIPO PCT/US2005/041532 11/15/2005    
Methods and Solutions for Tissue Preservation Divisional USA 14/530,006   10/31/2014    
Category(s):
Tool
For Information, Contact:
Julien Brohan
julien.brohan@cshs.org
Inventors:
Andreas Kamlot
Alfredo Trento
Lawrence Czer
Jennifer Pass
Keywords:
Cardiovascular