PTTG Knockout Rodent as a Model to Study Mechanisms for Various Physiological Phenomena, including Diabetes

Tech ID: mel000052

 

Introduction

Pituitary tumor transforming gene (PTTG) is a recently observed oncogene isolated from pituitary tumor growth hormone-secreting cells. Despite all of the research and resources applied to understanding the role of PTTG in cell cycle control and the pathogenesis of numerous disease conditions, including tumorigenesis, the function and mode of action of PTTG in vivo remains poorly understood. This is due, in part, to the fact that there has been no readily available and effective in vivo model for studying PTTG.

 

Technology Description

Disclosed is a null mutant (or knockout) rodent comprising, in its germ cells, an artificially induced PTTG null mutation. In some embodiments, the null mutant rodent can be generated by way of homologous recombination in an embryonic stem cell or germ cell. The inventive null mutant rodent can be used to study mammalian physiology at the cellular, tissue, and/or organismal level with respect to various phenotypes, including hyperglycemia, hypoinsulinaemia, hypoleptinemia, diabetes, chromosomal aneuploidy, premature centromere division, chromosomal damage, aberrant mitotic cellular division, thrombocytopenia, thymic hyperplasia, splenic hypoplasia, testicular hypoplasia, and female subfertility. Also disclosed is an animal model for diabetes, a somatic or germ cell obtained from the null mutant rodent, and a cell line derived from a cell obtained from the null mutant rodent.

 

Stage of Development

Knockout mouse model generated.

 

Applications

The mouse model can be used to study PTTG gene related diseases.

 

Intellectual Property

US patent 6,909,030 granted

Patent Information:
Category(s):
Tool
For Information, Contact:
Wenyue Du
Senior Associate - IP Management & Licensing
310-423-2241
wenyue.du@cshs.org
Inventors:
Shlomo Melmed
Zhiyong Wang
Keywords:
Research