L-Glass: a novel functionalization method for covalently attaching ECM Protein to optical glass

Tech ID: lap001074

Introduction

L-Glass is a method to treat optical glass and coat with extracellular matrix (ECM) protein. Compared to conventional coating methods, this new method allows the ECM protein to resist degradation and prevent cell detachment over extended culture period. Therefore, the coated glass vessels are superior for long period studies such as neuron stem cell development.   

 

Technology Description

Extracellular matrix (ECM) proteins such as collagen, fibronectin, and laminin are major components known to regulate cellular phenotypes during stem cell development. Treating glass and coating with ECM proteins allow for cell attachment and study of cellular development in a complex and dynamic environment. In addition, the thin and transparent nature of glass allows observation of cells using optical techniques in contrast to plastic which is autofluorescent and not optically clear. Examples of existing glass treatment methods employ poly-L-orthinine (PLO), poly-L-Lysine (PLL), or silane reagents. Although glass treated with these methods shows good short-term performance, significant ECM protein degradation and cell detachment are observed during extended culture period.

 

Dr. Laperle and Dr. Svendsen developed a new method, known as L-Glass, to treat glass and coat ECM proteins. Their results showed that by day 35, the iPSC derived dopaminergic neurons on PLO coated glass had fully clumped and largely detached, while neurons on L-Glass were attached and viable past day 50.

 

Applications

•       This invention can be developed into glass cell culture vessels. The product may be uncoated or coated with desired ECM protein before shipment.

 

Intellectual Property

PCT/US2018/042173 filed

 

 

 

 

Figure: Culture of iPSC derived dopaminergic neurons on L-Glass and PLO coated glass